Abstract:
Objective The genus Rhinogobius is highly diverse, exhibiting significant variation in morphological traits, which makes accurate species identification and classification challenging when using traditional morphological methods. Rhinogobius changtinensis, a species in this genus, lacks clear mitochondrial genomic data, and its phylogenetic relationships remain poorly understood. This study aims to analyze the mitochondrial genome of R. changtinensis to provide insights into species identification, classification, and the phylogenetic study of the genus Rhinogobius.
Methods Using the Illumina NovaSeq X Plus platform, we conducted next-generation sequencing on the mitochondrial genome of R. changtinensis, followed by the assembly and annotation of the genome sequence. We analyzed the structural features and base composition of the mitochondrial genome. Based on the nucleotide sequences of 13 protein-coding genes, we constructed a phylogenetic tree using Maximum Likelihood (ML) and Bayesian Inference (BI) methods.
Results The mitochondrial genome of R. changtinensis is 16 673 bp in length, comprising 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and an AT-rich region of 854 bp. The gene arrangement is consistent with most species within the genus. Phylogenetic analysis indicates that R. changtinensis is most closely related to Rhinogobius duospilus (PP=1, BS=100%).
Significance This study provides valuable mitochondrial genomic data for R. changtinensis and offers crucial insights into the phylogenetic relationships within the genus Rhinogobius, contributing to the understanding of its systematics.
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